Y2021
Yeast Synthetic Drop-out Medium Supplements
without histidine, leucine, tryptophan, and adenine
Synonym(s):
Yeast Synthetic Drop-out Media Supplements
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About This Item
Quality Level
form
powder
technique(s)
transformation: suitable
application(s)
microbiology
storage temp.
room temp
Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.
Preparation Note
Dissolve 1.39 g of supplement per liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y2021-20G | 04061837588006 |