Y1376
Yeast Synthetic Drop-out Medium Supplements
without leucine
Synonym(s):
Synthetic Yeast Growth Media
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Preparation Note
1.6 g will supplement 1 liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Amino acids: All standard amino acids except for leucine. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Still not finding the right product?
Explore all of our products under Yeast Synthetic Drop-out Medium Supplements
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Related Content
Instructions
Christopher J Wilkinson et al.
BMC cell biology, 10, 17-17 (2009-03-04)
The centrosome is the cell's microtubule organising centre, an organelle with important roles in cell division, migration and polarity. However, cells can divide and flies can, for a large part of development, develop without them. Many centrosome proteins have been
Tim Snoek et al.
Nucleic acids research, 48(1), e3-e3 (2019-11-30)
Allosteric transcription factors (aTFs) have proven widely applicable for biotechnology and synthetic biology as ligand-specific biosensors enabling real-time monitoring, selection and regulation of cellular metabolism. However, both the biosensor specificity and the correlation between ligand concentration and biosensor output signal
Renata Novakova et al.
Applied microbiology and biotechnology, 102(2), 857-869 (2017-12-03)
Mithramycin A is an antitumor compound used for treatment of several types of cancer including chronic and acute myeloid leukemia, testicular carcinoma, hypercalcemia and Paget's disease. Selective modifications of this molecule by combinatorial biosynthesis and biocatalysis opened the possibility to
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y1376-20G | 04061837587887 |