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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.53
EC Number:
232-667-0
MDL number:
Specific activity:
10000 units/mg protein
Biological source:
bovine pancreas
Concentration:
5—15 mg protein/mL
biological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
buffered aqueous glycerol solution
specific activity
10000 units/mg protein
mol wt
29.1 kDa
concentration
5—15 mg protein/mL
technique(s)
DNA extraction: suitable
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
foreign activity
RNase, none detected
storage temp.
−20°C
Gene Information
cow ... DNASE1(282217)
General description
Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Application
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.
Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Other Notes
DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
Articles
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
Related Content
S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D7291-.5MG | 04061833588376 |
| D7291-2MG | 04061833588383 |
