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D2916

Lambda DNA Mixed Digest

Name: Lambda DNA Mixed Digest
Brand: SIGMA

for DNA electrophoresis

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About This Item

NACRES:

NA.25

UNSPSC Code:

12352200

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Properties

Quality Level

100, 200

form

buffered aqueous solution

usage

100 uses (0.1 mg size), 20 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Description

General description

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Preparation Note

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.4%.

Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Global Trade Item Number

SKU	GTIN
D2916-20UG	04061833562697
D2916-.1MG	04061833562680