D0672
φX174 DNA Marker Hae III Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Storage temp.:
−20°C
General description
Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Preparation Note
φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Articles
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D0672-20UG | 04061835572465 |
| D0672-.1MG | 04061833557969 |