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93290

TRIS borate – EDTA buffer solution

for Northern and Southern blotting, solution

Synonym(s):

TBE buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25
eCl@ss:
32129211
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grade

Molecular Biology

Quality Level

100

description

long run, concentrate

product line

BioReagent

form

solution

quality

10× concentrate, ready-to-use solution

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.6-9.0

storage temp.

room temp

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Preparation Note

The TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
1401336
1401332
1385086
1365123
1365126

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

L. Johnston-Dow et al.
Biotechniques, 5, 754-754 (1987)

Global Trade Item Number

SKUGTIN
93290-1L04061833282564
93290-5L04061833282571