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57180

Indo 1

suitable for fluorescence, ≥80.0% (HPLC)

Synonym(s):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt

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About This Item

Empirical Formula (Hill Notation):
C32H26K5N3O12
CAS Number:
132319-56-3
Molecular Weight:
840.05
NACRES:
NA.32
PubChem Substance ID:
329758276
UNSPSC Code:
12352200
MDL number:
MFCD00077797
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assay

≥80.0% (HPLC)

form

solid

fluorescence

λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+, λex 349 nm; λem 482 nm (100mM KCI)

suitability

in accordance for fluorescence, suitable for fluorescence

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

InChI key

VGZPRMSXVNDMAN-UHFFFAOYSA-I

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Other Notes

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Microsphere-based real-time calcium sensing.
Rosario M Sánchez-Martín et al.
Angewandte Chemie (International ed. in English), 45(33), 5472-5474 (2006-07-19)
M K Bijsterbosch et al.
Biochemical and biophysical research communications, 137(1), 500-506 (1986-05-29)
The new Ca2+-probe indo-1 has a high fluorescence intensity, which allows low intracellular dye loadings. Stimulation of indo-1-loaded mouse B cells with anti-Ig antibodies provoked rapid rise of free cytoplasmic Ca2+ from 100 nM to greater than 1 microM, followed
Michael Bannwarth et al.
ACS chemical biology, 4(3), 179-190 (2009-02-06)
The role of calcium in signal transduction relies on the precise spatial and temporal control of its concentration. The existing means to detect fluctuations in Ca2+ concentrations with adequate temporal and spatial resolution are limited. We introduce here a method
View All Related Papers

Global Trade Item Number

SKUGTIN
57180-1MG-F04061823354684
57180-5MG-F04061832532691