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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
eCl@ss:
32160410
Specific activity:
~0.8 U/mg
Biological source:
Aspergillus niger
biological source
Aspergillus niger
Quality Level
form
powder
specific activity
~0.8 U/mg
color
off-white
storage temp.
2-8°C
General description
Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.
Application
Cellulase from Aspergillus niger has been used:
- in the preparation of the standardized solution to determine absolute cellulase levels
- in phosphate-citric acid buffer for activity assay
- in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed
Biochem/physiol Actions
Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
Other Notes
1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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M.A. Sawsan et al.
Zentralblatt Fur Mikrobiologie, 143, 55-55 (1988)
G. Okada
Methods in Enzymology, 160, 259-259 (1988)
María Dolores Ayala et al.
Animals : an open access journal from MDPI, 10(12) (2020-12-05)
A 90-d feeding trial was conducted in which five groups of gilthead seabream (11.96 g initial body weight) were fed with a microalgae-free diet (control group, C) or four diets containing the microalgae Nannochloropsis gaditana at two inclusion levels (2.5%
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 22178-25G | 04061838777560 |
| 22178-100G | 04061838777553 |
| 1054065000 | 04022536775401 |
| 1054060500 | 04022536064246 |
