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MilliporeSigma

12306C

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonym(s):

HI FBS, HI FCS, HI Fetal Bovine Serum, HI Fetal Calf Sera, Heat Inactivated Fetal Calf Sera, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered

Key Documents

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Premier

quality

from USDA approved countries

composition

Hemoglobin, ≤25 mg/dL

origin

non-USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

−20°C

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Application

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.
USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
17L186
14E314H1
13G114H1
12C483H1

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Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
Asgari S, et al.
Stem Cell Reviews and Reports, 9(4), 493-504 (2013)
Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
Vosough M, et al.
Stem Cells and Development, 22(20), 2693-2705 (2013)
Gerhard Gstraunthaler
ALTEX, 20(4), 275-281 (2003-12-13)
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The
Xiao-Min Liu et al.
Nature chemical biology, 15(9), 865-871 (2019-08-07)
RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A
Michael P Collins et al.
The Journal of biological chemistry, 295(28), 9433-9444 (2020-05-16)
The vacuolar H+-ATPase (V-ATPase) is an ATP-dependent proton pump that is essential for cellular homeostasis. V-ATPase activity is controlled by the regulated assembly of the enzyme from its component V1 and V0 domains. We previously reported that amino acid starvation
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Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Protocols

Freezing and Thawing FBS and Other Sera

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Fetal Bovine Serum Sourced in USDA Approved Countries heat inactivated
Suero bovino fetal que da la talla.

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