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MilliporeSigma

C2432

Sigma-Aldrich

Chloroform

contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Synonym(s):

Methylidyne trichloride, Trichloromethane

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About This Item

Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein/REAXYS Number:
1731042
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.04
Assay:
≥99.5%
Technique(s):
RNA extraction: suitable
Bp:
60.5-61.5 °C (lit.)
Vapor pressure:
160 mmHg ( 20 °C)
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vapor density

4.1 (vs air)

Quality Level

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

contains

100-200 ppm amylenes as stabilizer

technique(s)

RNA extraction: suitable

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. The product contains amylene as a stabilizer, since chloroform is susceptible to degradation with time. Amylene acts as a scavenger of free radicals formed during chloroform decomposition.
Our biotech solvents offer exceptional quality for reliable RNA extraction, ensuring optimal laboratory performance. With low water content and minimal residue, give clean UV spectra to minimize contamination.
  • High-Quality Biotech Solvents: Designed for optimal laboratory performance with low water content and minimal residues.
  • Ideal for RNA Extraction: Essential for genetic testing and research applications like PCR.
  • Ensures Integrity of Genetic Material: Promotes reliable and reproducible results.
  • Industry Compliant: Suitable for both academic and commercial laboratories, enhancing research efficiency.

Application

Chloroform has been used for the isolation of RNA from mammalian tissue.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash


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RNA Isolation from Synechocystis.
Mironov KS and. Los DA.
Bio-protocol, 5(6) (2015)
Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells.
Ramos P et al.
Respiratory Research, 15, 54-54 (2014)
GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species.
GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species.
Kosma DK, et al.
Bio-protocol, 5(24) (2015)
Immune activation and target organ damage are consequences of hydrodynamic treatment but not delivery of naked siRNAs in mice.
Racz Z et al.
Nucleic Acid Therapeutics, 21, 215-215 (2011)
Min Wang et al.
Journal of colloid and interface science, 445, 84-92 (2015-01-18)
Phospholipids fulfill an important role in joint lubrication. They, together with hyaluronan and glycoproteins, are the biolubricants that sustain low friction between cartilage surfaces bathed in synovial fluid. In this work we have investigated how the friction force and load

Articles

Substances are said to be miscible in one another if they dissolve to form a uniform solution. Bookmark or download our miscibility table for common lab solvents.

Se dice que las sustancias son miscibles entre sí si se disuelven hasta formar una disolución uniforme. Marque o descargue nuestra tabla de miscibilidad para los disolventes comunes del laboratorio.

Protocols

Preparation for biodegradable nanoparticles and their use in transfection protocols .

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