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MilliporeSigma

61768

Lectin, immobilized on Agarose CL-4B from Triticum vulgaris

suitable for affinity chromatography

Synonym(s):

Wheat germ agglutinin, immobilized on agarose CL-4B

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About This Item

NACRES:
NA.21
UNSPSC Code:
12190000
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technique(s)

affinity chromatography: suitable

Quality Level

capacity

0.1-1.5 mg, packed gel capacity (ovomucoid/ml)

storage temp.

2-8°C

Physical form

50% aqueous suspension in 1 M NaCl

Other Notes

Protein isolation from bordetella pertussis that facilitates entry of calmodulin-sensitive adenylate cyclase into animal cells


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Protocols

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

This page shows how to prepare samples for purification with affinity chromatography.

View All Protocols

Articles

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.


M G Donovan et al.
Biochemistry, 28(20), 8124-8129 (1989-10-03)
Bordetella pertussis, the pathogen responsible for whooping cough, releases a soluble calmodulin-sensitive adenylate cyclase into its culture medium. Several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular cAMP levels [Confer



Global Trade Item Number

SKUGTIN
61768-1ML04061838210760
61768-5ML04061838196187