11663925910
Roche
Mycoplasma PCR ELISA
usage
sufficient for 96 reactions
manufacturer/tradename
Roche
shipped in
dry ice
storage temp.
−20°C
Application
For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.
Biochem/physiol Actions
The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.
Features and Benefits
Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
- High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
- Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
- Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
- Fast results: The entire procedure can be performed in one day
- Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing
Packaging
1 kit containing 14 components.
Other Notes
Chain Length Positive control: 520 bp.
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Lysis Reagent
- Neutralization Reagent
- PCR Mix ready-to-use
- Denaturation Reagent
- Hybridization Buffer
- Biotinylated Capture Probe
- Washing Buffer 10x concentrated
- Anti-Digoxigenin-POD antibody
- Conjugate Dilution Buffer
- TMB Substrate Solution
- Stop Reagent
- Positive Control DNA
- Streptavidin-coated Microplate
- Adhesive Plate Cover Foils
See All (14)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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