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11051440001

Roche

dATP

Name: dATP
Brand: ROCHE

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

Synonym(s):

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 2′-deoxy-adenosine-5′-triphosphate, deoxy-adenosine-5′-triphosphate, 2′-, dATP

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About This Item

UNSPSC Code:

41116100

NACRES:

NA.54

Assay:

98%

Form:

solution

Storage temp.:

−20°C

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Properties

description

Lithium salt

assay

98%

form

solution

usage

sufficient for 6,250 standard reactions (PCR)

packaging

pkg of 250 μL (25 μmol; 100 mM)

manufacturer/tradename

Roche

concentration

100 mM

technique(s)

DNA amplification: suitable, DNA sequencing: suitable, DNA synthesis: suitable, PCR: suitable

storage temp.

−20°C

SMILES string

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

Description

General description

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.

dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

dATP has been used to resume transcription.

Biochem/physiol Actions

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro. dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.

Analysis Note

Typical analysis:

≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
DNase activity negative, according to the current Quality Control procedures
Suitability for PCR

Other Notes

1 μmol dATP = 15.0 A₂₆₀ units

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

nucleotide sets (all 4 nucleotides in separate vials)
ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

kits (dNTPs, buffer, and polymerase in separate vials)
ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

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flash_point_c

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Global Trade Item Number

SKU	GTIN
11051440001	04061838697509