10109886001
Roche
Trypsin Inhibitor
from soybean
Synonym(s):
trypsin
biological source
soybean
Quality Level
form
lyophilized
mol wt
Mr 20,100
packaging
pkg of 50 mg
manufacturer/tradename
Roche
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
pH
3.8-4.5
solubility
water: soluble
storage temp.
2-8°C
General description
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.
Application
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- in the buffer for hepatocyte isolation
- to inhibit trypsin in Corneal epithelial cells
- to inhibit papain activity while treating hippocampal neural stem cells (NSCs)
Biochem/physiol Actions
Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.
Features and Benefits
Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)
Isoelectric point: 4.5
Isoelectric point: 4.5
Preparation Note
Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Sensitive to heat, alkaline pH, and protein-precipitating compounds.
Store at 2 to 8 °C. (Store dry!)
Other Notes
1 U = 270 S & T-units or 90 USP-units
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Effects of soybean trypsin inhibitor on hemostasis
Soybean-Bio-Active Compounds (2013)
Mice, double deficient in lysosomal serine carboxypeptidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular corneal dystrophy and hypertrophic skin thickenings
Pan X, et al.
PLoS ONE, 12(2), e0172854-e0172854 (2017)
proBDNF is modified by advanced glycation end products in Alzheimer?s disease and causes neuronal apoptosis by inducing p75 neurotrophin receptor processing
Fleitas C, et al.
Molecular Brain, 11(1), 68-68 (2018)
Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid-Overlay Technique
Kyffin JA, et al.
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-chief) ... [Et al.], 81(1), e87-e87 (2019)
Isolating Taste Buds and Taste Cells from Vallate Papillae of C57BL/6J Mice for Detecting Transmitter Secretion
Anthony Y. Huang and Sandy Y. Wu
CorpBase ID (for auto-filling citation data), 6(11) (2016)
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