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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Biological source:
bovine pancreas
biological source
bovine pancreas
form
lyophilized
packaging
pkg of 100 mg
manufacturer/tradename
Roche
technique(s)
DNA extraction: suitable
optimum pH
~7.0
storage temp.
2-8°C
General description
Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Application
Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
- The isolation of cells from lung, skin and tumor samples
- Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †
Preparation Note
Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10104159001 | 04061838250445 |