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MilliporeSigma

WBAVDP001

Millipore

Immobilon® Block - PO (Phosphoprotein Blocker)

Western blotting, dot/slot blotting, mass spectrometry, phosphorolation assay

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

Key Documents

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Quality Level

300

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent (phosphoprotein)
fluorometric (phosphoprotein)

shipped in

ambient

storage temp.

15-25°C

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Preparation Note

Stable for 1 year at 20–25°C

Analysis Note

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts

Articles

Immunodetection

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

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