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MilliporeSigma

TMS-010

Sigma-Aldrich

Alcian-Blue Staining Solution

Synonym(s):

Alcian blue 8G, Alcian blue PAS stain, Alcian blue stain

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Technical Service
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technique(s)

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

Quality Level

100

Related Categories

General description

Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Physical form

Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.

Preparation Note

Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Analysis Note

Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

FlameCorrosion
GHS02,GHS05

signalword

Danger

hcodes

H226,H314

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

102.2 °F - closed cup

flash_point_c

39 °C - closed cup

Certificates of Analysis (COA)

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Articles

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Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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