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MilliporeSigma

TMS-009

Sigma-Aldrich

Safranin-O Staining Solution

Safranin-O is often used to identify chondrocytes that have been derived from both human and rodent mesenchymal stem cells (MSCs).

Synonym(s):

Basic Red 2

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Quality Level

100

concentration

0.1 % (w/v) in distilled water

technique(s)

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

Related Categories

General description

Safranin-O, also known as basic red 2, is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring all cell nuclei red. It can also be used for the detection of cartilage, mucin and mast cell granules. Safranin-O is often used to identify chondrocytes that have been derived from both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Physical form

Product is presented as a 0.1% w/v solution diluted in distilled water.

Preparation Note

Store at room temperature for up to 4 months from date of receipt. If Safraninin-O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Analysis Note

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Articles

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Protocols

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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