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MilliporeSigma

SCNY00100

Millipore

Millipore® Steriflip® Vacuum Tube Top Filter

pore size 100.0 μm, Nylon membrane (net), funnel capacity 50 mL, pack of 25 ea

Synonym(s):

Steriflip® Nylon Net Sterile Centrifuge Tube Top Filter Unit, tube top sterile filter unit, tube top vacuum filter, tube top vacuum sterile filtering unit

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About This Item

UNSPSC Code:
41104914
eCl@ss:
32031602
NACRES:
NB.22
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material

Nylon membrane (net)
acrylic housing
polypropylene

Quality Level

sterility

sterile

product line

Steriflip®

feature

holdup volume 0.6 mL
hydrophilic

packaging

pack of 25 ea

parameter

45 °C max. temp.

technique(s)

sterile filtration: suitable

H

138 mm

diam.

6.4 cm

filter diam.

4 cm

filtration area

7 cm2

funnel capacity

50 mL

working volume

50 mL

pore size

100.0 μm pore size

fitting

double lead thread inlet (with vacuum port)
double lead thread outlet for 50 mL centrifuge tube

shipped in

ambient

General description

Steriflip® filtration devices allow the separation of larger volumes of cellular material with the added advantage of maintaining a sterile environment ensuring sample sterility, an option that open-top gravity flow filter devices cannot guarantee. Steriflip Sterile Centrifuge Tube Top Filter Unit permits the passage of cells while retaining large tissue clumps. This improves recovery and reduces the time to collect the isolated cells.

Application

For Research Use Only.
Steriflip® Sterile Centrifuge Tube Top Filter Unit has been used in tumor procurement and processing. It has also been used in the isolation of cardiac stem cells (CSCs) from the extracted hearts of Balb/c mice.

Legal Information

Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Steven D Forsythe et al.
Annals of surgical oncology, 27(13), 4950-4960 (2020-07-08)
Chemotherapy dosing duration and perfusion temperature vary significantly in HIPEC protocols. This study investigates patient-derived tumor organoids as a platform to identify the most efficacious perfusion protocol in a personalized approach. Peritoneal tumor tissue from 15 appendiceal and 8 colon

Articles

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

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Related Content

The isolation and expansion of cardiac stem cells opens new opportunities to the feld of cardiac regenerative medicine. CSCs have recently been isolated from human and murine tissues based upon cell biomarkers (Sca-1, TERT, c-Kit, side population), demonstrating the presence of a non-circulating stem cell niche within the myocardium that is estimated to account for 11-14% of the total cell population1. These cells appear to be bi-potent in their capacity to form cardiomyocytes and vascular endothelial cells. Moreover, preliminary engraftment studies suggest that these cells are ideal candidates for future research on cardiac regeneration. However, the rarity of CSCs coupled with the complex isolation procedure is a signifcant challenge for the advancement of the field. o overcome this obstacle, we have developed an easy-to-use cell isolation kit capable of obtaining a high yield, pure population of CSCs. This advancement allows investigators to obtain a signifcantly greater number of CSCs for their studies without the need for time consuming, complex protocols and expensive cell sorting equipment.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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