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MilliporeSigma

SCM130

Sigma-Aldrich

PluriSTEM® Human ES/iPS Cell Medium

Defined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.

Synonym(s):

Human Embryonic Stem Cell Media, Human iPS Cell Media, Pluripotent Stem Cell Media

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Key Documents

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Quality Level

100

form

liquid

manufacturer/tradename

PluriSTEM®

technique(s)

cell culture | embryo: suitable
cell culture | stem cell: suitable

shipped in

dry ice

Related Categories

General description

EMD Millipore has developed a complete, defined and serum-free medium formulation for the feeder-free culture of human ES and iPS cells. PluriSTEM Human ES/iPS Cell (Cat. No. SCM130) is a small molecule based medium that enables weekend-free culture of human pluripotent stem cells and allows for media exchanges every other day without compromising the morphology or long term functionality of pluripotent stem cells. Pluripotent cells maintained in this moderate feeding regiment exhibited high cell health with minimal spontaneous differentiation,; expressed high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retained differentiation potential and possessed a normal karyotype. PluriSTEM Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation. Cells proliferate faster in PluriSTEM as compared to the same cells plated in other serum-free and feeder-free media systems; a typical 4-6 day passage is expected for most pluripotent cell lines.
PluriSTEM has been extensively tested and proven to possess the following characteristics for Human ES/iPS cell culture:

• Supports the culture of pluripotent human ES/iPS cells for greater than 30 passages

• Eliminates the requirement to feed cells over the weekend. Take the weekend off.

• Eliminates the requirement of daily feeding (i.e. Monday, Wednesday, Friday media exchanges).

• Simple and easy transition of cells to PluriSTEM from feeder-based and feeder-free culture systems.

• Enables superior single cell culture, passage and selection of human pluripotent clones.

• Superior freeze-thaw cell viability and recovery.

• Supports suspension culture.

Preparation Note

PluriSTEM Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on product label. Before use, thaw PluriSTEM at room temperature or overnight at 2-8°C. Do not thaw at 37°C. If desired, PluriSTEM may be aseptically dispensed into working aliquots and stored at -20°C. Use aliquots within expiration date as indicated on label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.

Analysis Note

Each lot of PluriSTEM® Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.
pH= 7.2-7.4
Osmolality= 315-345
Appearance= Red Clear Liquid

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human pluripotent stem cells on artificial microenvironments: a high content perspective.
Viswanathan, P; Gaskell, T; Moens, N; Culley, OJ; Hansen, D; Gervasio, MK; Yeap, YJ; Danovi, D
Frontiers in Pharmacology null
Doxycycline enhances survival and self-renewal of human pluripotent stem cells.
Chang, MY; Rhee, YH; Yi, SH; Lee, SJ; Kim, RK; Kim, H; Park, CH; Lee, SH
Stem Cell Reports null
Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells.
Kadari, Asifiqbal, et al.
Stem Cell Research & Therapy, 5, 47-47 (2014)

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In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

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Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

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Protocols

Passaging Human iPS Cells Protocol Using EZ-LiFT™ Reagent

Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Related Content

Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.

PluriSTEM™ Human ES/iPS Medium

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