SCC058
FibroGRO® Xeno-Free Human Foreskin Fibroblasts
The FibroGRO Xeno-Free Human Foreskin Fibroblasts are derived from normal human foreskin & have been isolated & manufactured under xeno-free conditions.
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About This Item
biological source
human foreskin (fibroblasts)
Quality Level
packaging
pkg of 1 kit
manufacturer/tradename
Chemicon®, FibroGRO®, Millipore
morphology
(endothelial)
technique(s)
cell culture | mammalian: suitable, cell culture | stem cell: suitable
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
Application
Stem Cell Research
Biochem/physiol Actions
Preparation Note
Analysis Note
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Legal Information
Disclaimer
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Articles
Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.
Simplicon™ RNA Reprogramming generates human iPS cells using synthetic, polycistronic self-replicating RNA strands mimicking cellular RNA.
Related Content
Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCC058 | 04053252650567 |