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MilliporeSigma

SCC034

Sigma-Aldrich

Human Mesenchymal Stem Cells (Bone Marrow)

Human mesenchymal stem cells derived from bone marrow that have a capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes.

Synonym(s):

Human Bone Marrow MSCs, Human Bone Marrow Multipotent Stem Cells, Human MSCs

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About This Item

UNSPSC Code:
41106509
eCl@ss:
32011203
NACRES:
NA.75

Key Documents

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biological source

human

Quality Level

100

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

input

sample type mesenchymal stem cell(s)

shipped in

liquid nitrogen

Related Categories

General description

Mesenchymal stem cells, also known as marrow stromal cells, are defined as a self-renewing population of adherent, bone-marrow-derived multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined cultures in vitro, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissues.

EMD Millipore’s Human Mesenchymal Stem Cells (Bone Marrow) are isolated from normal human bone marrow and are cryopreserved at P2 to ensure optimal phenotype and the highest viability and plating efficiency. These cells proliferate as an adherent cell monolayer and have shown a strong capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes..

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Mesenchymal Stem Cells

Preparation Note

Human Mesenchymal Stem Cells (Bone Marrow) should be stored in liquid nitrogen. The cells are guaranteed for at least 3 population doublings without significantly affecting cell surface marker expression and differentiation potential.

Analysis Note

- Each vial contains at least 1X10⁶ viable cells at P2.
- Cell viability is greater than 75% at thaw and cells can be cultured for at least 3 population doublings after the initial thaw.
- Bone marrow donors tested negative for HIV, hepatitis B and hepatitis C.
- Apparently normal karyotype per cytogenetic analysis.
- Cells tested negative for mycoplasma contamination.

Other Notes

1X106 viable Human Bone Marrow Mesenchymal Stem Cells

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Diego Omar Sanchez Ramirez et al.
Bioengineering (Basel, Switzerland), 8(12) (2021-12-24)
Protein-based nanofibres are commonly used in the biomedical field to support cell growth. For this study, the cell viability of wool keratin-based nanofibres was tested. Membranes were obtained by electrospinning using formic acid, hexafluoroisopropanol, and water as solvents. For aqueous
Christy Wing Tung Wong et al.
Stem cell research & therapy, 13(1), 419-419 (2022-08-14)
Critical limb ischemia (CLI) is the most severe form of peripheral artery disease and exhibits a high risk of lower extremity amputations. As even the most promising experimental approaches based on mesenchymal stem cells (MSCs) demonstrated only moderate therapeutic effects
LncRNA-NEF is downregulated in postmenopausal osteoporosis and is related to course of treatment and recurrence.
Xiaoyong Ma et al.
The Journal of international medical research, 47(7), 3299-3306 (2019-06-22)
Adam R Wolfe et al.
Oncotarget, 7(50), 82482-82492 (2016-10-21)
Inflammatory breast cancer (IBC) is a unique and deadly disease with unknown drivers. We hypothesized the inflammatory environment contributes to the IBC phenotype. We used an in vitro co-culture system to investigate interactions between normal and polarized macrophages (RAW 264.7
Edo Dzafic et al.
BioMed research international, 2014, 508216-508216 (2014-04-12)
After removal of oocytes for in vitro fertilization, follicular aspirates which are rich in somatic follicular cells are discarded in daily medical practice. However, there is some evidence that less differentiated cells with stem cell characteristics are present among aspirated
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Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

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PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

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Protocols

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells

We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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