MRCF0R030
Microcon® Centrifugal Filters
NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL
Synonym(s):
Centrifuge concentrator
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About This Item
material
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
Quality Level
sterility
non-sterile
feature
holdup volume> 10 μL
packaging
pkg of 100 ea
manufacturer/tradename
Microcon®
parameter
0.5 mL sample volume
technique(s)
DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable
L
4.50 cm (1.8 in.)
diam.
12.3 mm
filtration area
0.32 cm2
working volume
0.5 mL
pore size
30 kDa NMWCO
shipped in
ambient
Related Categories
General description
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X
Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing
Sample applications:
•Recovery of genomic DNA for forensic applications
•Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
•Removal of primers from amplified DNA
•Easy filtrate recovery, exploiting the flat membrane design
Other Notes
Legal Information
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
Protocols
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
Related Content
The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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