MR-101
EmbryoMax® Advanced KSOM Embryo Medium
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
Synonym(s):
K+ Simplex Optimised Medium (KSOM), KSOM Mouse Embryo Media
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About This Item
Quality Level
form
liquid
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | embryo: suitable
cell culture | stem cell: suitable
input
sample type: mouse embryo(s)
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General description
Traditionally, retrieving and then growing mouse embryos in vitro is usually a 2-step system. Embryos are commonly removed from the oviducts in a HEPES buffered medium (e.g. M2, FHM), which maintain their pH in air. The embryos are then transferred into a second medium which is bicarbonate buffered (e.g. M16, CZB, KSOM) and placed in a CO2 incubator in order to maintain the pH of the medium. Furthermore, the bicarbonate buffered medium needs to be pre-equilibrated in the incubator for several hours prior to receiving embryos to achieve optimal growth. The EmbryoMax Advanced KSOM Medium enables both embryo handling in atmospheric conditions and culturing in a CO2 incubator with a single medium. No medium pre-equilibration step is necessary for embryo harvest, but pre-equilibration is recommended for culture.
Application
Stem Cell Research
Preparation Note
Legal Information
Disclaimer
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
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