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MilliporeSigma

MAB363

Sigma-Aldrich

Anti-NMDAR1 Antibody, clone 54.1

clone 54.1, Chemicon®, from mouse

Synonym(s):

N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

54.1, monoclonal

species reactivity

rat, monkey, human, Xenopus

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable

isotype

IgG2a

NCBI accession no.

NM_000832.5

UniProt accession no.

Q05586

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

frog ... Grin1(780048)
human ... GRIN1(2902)
rat ... Grin1(24408)
rhesus monkey ... Grin1(574380)

General description

103-116 kDa
Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.

Immunogen

Epitope: Extracellular loop between transmembrane regions III & IV.
Recombinant fusion protein containing GST and NMDAR1 aa. 660-811.

Application

Anti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.
Immunocytochemistry:
A previous lot of this antibody was used in IC.

RIA:
A previous lot of this antibody was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA.

Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.

Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Biochem/physiol Actions

Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat brain and spinal cord tissue
Evaluated by immunohistochemistry on rat brain and spinal cord tissue.

Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A rotating bluff-body disc for reduced variability in wind tunnel aerosol studies.
Koehler KA, Anthony TR, van Dyke M, Volckens J.
Annals of Occupational Hygiene null
Insect NMDA receptors mediate juvenile hormone biosynthesis.
Chiang, AS; Lin, WY; Liu, HP; Pszczolkowski, MA; Fu, TF; Chiu, SL; Holbrook, GL
Proceedings of the National Academy of Sciences of the USA null
N Sourial-Bassillious et al.
Neuroscience, 161(4), 1126-1134 (2009-04-14)
Bipolar disorder and schizophrenia are two debilitating mental health disorders associated with both severe impairment and increased suicide risk. Several lines of evidence indicate that these disorders are associated with disturbances in the glutamate system. For almost half a century
Stroke patients develop antibodies that react with components of N-methyl-D-aspartate receptor subunit 1 in proportion to lesion size.
Kalev-Zylinska, ML; Symes, W; Little, KC; Sun, P; Wen, D; Qiao, L; Young, D; During, MJ; Barber, PA
Stroke null
Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses.
E Tarusawa, K Matsui, T Budisantoso, E Molnar, M Watanabe, M Matsui, Y Fukazawa, R Shigemoto
The Journal of Neuroscience null
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Related Content

Pathways and Biomarkers of Glutamatergic Synapse Flyer

Glutamate is an excitatory neurotransmitter found in the synaptic vesicles of glutamatergic synapses. The post-synaptic neurons in these synapses contain ionotropic and metabotropic glutamate receptors. Glutamate binds to AMPA (α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid) subtype glutamate receptors, leading to sodium influx into the post-synaptic cell and resulting in neuronal excitability and synaptic transmission. The NMDA (N-methyl-d-aspartate) subtype glutamate receptors, on the other hand, regulate synaptic plasticity, and can influence learning and memory. The metabotropic g-protein coupled mGluRs modulate downstream calcium signaling pathways and indirectly influence the synapse’s excitability. The synaptic architecture includes intracellular scaffolding proteins (PSD-95, GRIP), intercellular cell adhesion molecules (NCAMs, N-Cadherins), and a variety of signaling proteins (CaMKII/PKA, PP1/PP2B). Processes critical for synaptic transmission and plasticity are influenced by these molecules and their interactions. When the function of these molecules is disrupted, it leads to synaptic dysfunction and degeneration, and can contribute to dementia as seen in Alzheimer’s disease.

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