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MAB1946-IM

Anti-Nidogen 1 Antibody, clone ELM1

Name: Anti-Nidogen 1 Antibody, clone ELM1
Brand: MM

clone ELM1, from rat

Synonym(s):

Nidogen-1, NID-1, Entactin

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

ELM1, monoclonal

Application:

ICC, IHC, WB

Citations:

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Properties

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

ELM1, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_035047

UniProt accession no.

P10493

target post-translational modification

unmodified

Gene Information

mouse ... Nid1(18073)

Description

General description

136.54 kDa calculated.

Nidogen-1 (UniProt: P10493; also known as NID-1, Entactin) is encoded by the Nid1 (also known as Ent) gene (Gene ID: 18073) ins murine species. Nidogen-1 is a highly conserved sulfated monomeric glycoprotein that is widely distributed in basement membranes and tightly associates with laminin. It is post-translationally N- and O-glycosylated. It is synthesized with a 28 aa signal peptide that is subsequently cleaved to produce the mature form. Nidogen -1 contains six EGF-like domains, four LDL-receptor class B repeats, one NIDO domain, one nidogen G2 barrel domain, and one thyroglobulin type 1 domain. Nidogen-1 shares about 50% homology with Nidigen-2 and shares similar adhesive properties. There is also a high degree of homology between murine and human Nidogen-1. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

Immunogen

Purified protein from basement membranes of mouse Engelbreth-Holm-Swarm (EHS) tumor.

Application

Anti-Nidogen 1, clone ELM1, Cat. No. MAB1946-I, is a rat monoclonal antibodythat detects Nidogen-1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A representative lot detected Nidogen-1 in Western Blotting applications (Arends, F., et. al. (2015). PLoS One. 10(2):e0118090).

Immunocytochemistry Analysis: A representative lot detected Nidogen-1 in Immunocytochemistry applications (Kick, K., et. al. (2016). Arterioscler Thromb Vasc Biol. 36(12):2346-2357).

Immunohistochemistry Analysis: A representative lot detected Nidogen-1 in Immunohistochemistry applications (Chang, Y.T., et. al. (2016). J Clin Invest. 126(2):721-31).

Biochem/physiol Actions

Clone ELM1 is a rat monoclonal antibody that detects Nidogen-1 in murine species.

Physical form

Format: Purified

Analysis Note

Evaluated by Immunohistochemistry in mouse kidney.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Nidogen-1 in mouse kidney tissue.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c