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MilliporeSigma

MAB1585

Sigma-Aldrich

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2

culture supernatant, clone 5A3.2, Chemicon®

Synonym(s):

Anti-ATITHS, Anti-BRN3A, Anti-Oct-T1, Anti-RDC-1, Anti-brn-3A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

5A3.2, monoclonal

species reactivity

chicken, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_006237.3

UniProt accession no.

Q01851

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... POU4F1(5457)

General description

46 kDa

Immunogen

Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).
Epitope: POU-domain protein

Application

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.
Immunohistochemistry: 1:5-1:125

Western blot: 1:50-1:100

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis.
Badea, TC; Williams, J; Smallwood, P; Shi, M; Motajo, O; Nathans, J
The Journal of Neuroscience null
AML1/ETO proteins control POU4F1/BRN3A expression and function in t(8;21) acute myeloid leukemia.
Dunne J, Gascoyne DM, Lister TA, Brady HJ, Heidenreich O, Young BD
Cancer Research null
Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells.
Ronald S Goldstein,Oz Pomp,Irina Brokhman,Lina Ziegler
Methods in Molecular Biology null
A novel type of glial cell in the retina is stimulated by insulin-like growth factor 1 and may exacerbate damage to neurons and Muller glia.
Fischer, AJ; Scott, MA; Zelinka, C; Sherwood, P
Glia null
The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
Zelinka, CP; Scott, MA; Volkov, L; Fischer, AJ
Testing null
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