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CP35

Sigma-Aldrich

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

liquid, clone 7E3-1B8, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

Key Documents

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

7E3-1B8, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:2000)
Immunofluorescence (1:200, indirect)
Immunoprecipitation )

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Dlg4(29495)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.
Recognizes the ~95 kDa PSD95 protein.
This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Immunogen

Rat
recombinant, rat PSD95

Application


Immunoblotting (1:2000)
Immunofluorescence (1:200, indirect)
Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Brakeman, P.R., et al. 1997. Nature386, 284.
Hualing, D., et al. 1997. Nature386, 279.
Kennedy, M.B. 1997. Trends in Neurosci.20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol.7, 93.
Cho., K.-O., et al. 1992. Neuron9, 929.
Immunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95′s proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jessica L Cifelli et al.
The Journal of biological chemistry, 291(23), 11981-11992 (2016-03-30)
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