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ACK5010PA

Amicon® Pro Affinity Concentration Kit Protein A with 10kDa Amicon® Ultra-0.5 Device

Amicon Pro Affinity Concentration Kit Protein A - A Centrifugal Tool for purifying & concentrating Antibodies.

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About This Item

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)
Pore size:
10 kDa NMWCO
Working volume:
10 mL
Sterility:
non-sterile
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material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Pro

parameter

10 mL sample volume

technique(s)

buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration), protein purification: suitable (enrichment)

L

12 cm (4.7 in.)

diam.

2.97 cm

filtration area

1 cm2

working volume

10 mL

pore size

10 kDa NMWCO

shipped in

wet ice

General description

Filter Code: PLCGC
Filter Type: Ultrafiltration
This kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 10 kDa, 3 ml Protein A resin (Binding capacity: 20mg human IgG/ml agarose), 2 X 66ml Bind/Wash Buffer, 12 ml Elution buffer, 2.5 ml Neutralization buffer

Features and Benefits

Minimum Final Concentrate Volume: 15 µL

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

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Global Trade Item Number

SKUGTIN
ACK5010PA-B04055977151091