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MilliporeSigma

ABN462

Sigma-Aldrich

Anti-Dopamine D2 Receptor Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Dopamine D2 Receptor, D2R

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

technique(s)

immunohistochemistry: suitable

NCBI accession no.

NP_034207

UniProt accession no.

P61168

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Drd2(13489)

General description

50.9 kDa calculated
The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Immunogen

Epitope: cytoplasmic third intracellular loop
Recombinant protein corresponding to the cytoplasmic domain of mouse Dopamine D2 Receptor.

Application

Anti-Dopamine D2 Receptor Antibody is an antibody against Dopamine D2 Receptor for use in Immunohistochemistry.
Immunohistochemistry Analysis: A representative lot detected Dopamine D2 Receptor in WT mouse striatum tissue (Trifilieff, P., et al. (2013). Molecular Psychiatry. 18:102-1033).
Immunohistochemistry Analysis: A representative lot detected Dopamine D2 Receptor in wild-type and not in D2R knockout mouse sriatum tissue (Trifilieff, P., et al. (2011). BioTechniques. 50:111-118).
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Biochem/physiol Actions

This antibody binds to the cytoplasmic third intracellular loop.

Physical form

Affinity
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry in mouse striatum tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Dopamine D2 Receptor in mouse striatum tissue.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Increasing dopamine D2 receptor expression in the adult nucleus accumbens enhances motivation.
Trifilieff, P; Feng, B; Urizar, E; Winiger, V; Ward, RD; Taylor, KM; Martinez, D; Moore et al.
Molecular Psychiatry null
Ying Zhu et al.
BioTechniques, 68(3), 122-129 (2019-12-21)
Despite the controversy regarding the existence and physiological relevance of class A G protein-coupled receptor dimerization, there is substantial evidence for functional interactions between the dopamine D2 receptor (D2R) and the adenosine A2A receptor (A2AR). A2AR-D2R complexes have been detected in
Pierre Trifilieff et al.
BioTechniques, 51(2), 111-118 (2011-08-03)
The existence of G protein-coupled receptor (GPCR) dimers and/or oligomers has been demonstrated in heterologous systems using a variety of biochemical and biophysical assays. While these interactions are the subject of intense research because of their potential role in modulating
Benjamin Lassus et al.
Scientific reports, 8(1), 17461-17461 (2018-12-01)
Although the prefrontal cortex and basal ganglia are functionally interconnected by parallel loops, cellular substrates underlying their interaction remain poorly understood. One novel approach for addressing this issue is microfluidics, a methodology which recapitulates several intrinsic and synaptic properties of

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