Skip to Content
MilliporeSigma

AB15894

Sigma-Aldrich

Anti-Tbr2 Antibody

from chicken, purified by affinity chromatography

Synonym(s):

Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2, Tbr2

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

chicken

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgY

NCBI accession no.

NP_034266

UniProt accession no.

O54839

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EOMES(8320)

General description

Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2, Tbr2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. Tbr2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of Tbr2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of Tbr2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).
~72 and 74 kDa observed; 74.8 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: Transcription Activation Domain
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of mouse T-box brain protein 2 (Tbr2).

Application

Anti-Tbr2, Cat. No. AB15894, is a chicken polyclonal antibody that detects Tbr2 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Tested Applications

  • Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in Mouse brain tissue sections.
  • Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

This chicken polyclonal antibody specifically detects T-box brain protein 2 (Tbr2). It targets an epitope within 20 amino acids from the C-terminal region.

Physical form

Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Recommended storage: +2°C to +8°C.

Analysis Note

Control
E13-14 mouse brain lysate
Evaluated by Western Blotting in E13-E14 Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Marta Florio et al.
Science advances, 2(12), e1601941-e1601941 (2016-12-14)
The gene ARHGAP11B promotes basal progenitor amplification and is implicated in neocortex expansion. It arose on the human evolutionary lineage by partial duplication of ARHGAP11A, which encodes a Rho guanosine triphosphatase-activating protein (RhoGAP). However, a lack of 55 nucleotides in
Mareike Albert et al.
The EMBO journal, 36(17), 2642-2658 (2017-08-03)
The generation of neocortical neurons from neural progenitor cells (NPCs) is primarily controlled by transcription factors binding to DNA in the context of chromatin. To understand the complex layer of regulation that orchestrates different NPC types from the same DNA
Natalie E Patzlaff et al.
Human molecular genetics, 26(7), 1340-1352 (2017-02-17)
Fragile X related protein 1 (FXR1P) is a member of the fragile X family of RNA-binding proteins, which includes FMRP and FXR2P. Both FMRP and FXR2P regulate neurogenesis, a process affected in a number of neurological and neuropsychiatric disorders, including
Anna Gorelik et al.
Nature communications, 8, 15096-15096 (2017-05-04)
In recent years the notion that malfunctioning of the immune system may result in developmental brain diseases has emerged. However, the role of immune molecules in the developing brain has not been well explored. The complement pathway converges to cleave
Elisa Penna et al.
Cerebral cortex communications, 2(4), tgab053-tgab053 (2021-10-15)
Microglial cells, the innate immune cells of the brain, are derived from yolk sac precursor cells, begin to colonize the telencephalon at the onset of cortical neurogenesis, and occupy specific layers including the telencephalic proliferative zones. Microglia are an intrinsic
View All Related Papers

Related Content

Neuroscience Solutions for productive research

Alzheimer’s Disease is a progressively deteriorating disease. It manifests itself with memory loss, confusion, problems with judgment, planning, concentration, and personality changes; and in it’s later stages, a decline in physical abilities. The disease’s causes, cures, and preventions are unknown; however, key proteins likely involved in the degenerative mechanism have been identified. Alzheimer’s Disease is characterized by neuronal loss, alterations in neurotransmitter systems, and the presence of neurofibrillary tangles composed of abnormally hyperphosphorylated tau proteins. A prominent feature of Alzheimer’s Disease is the formation of senile plaques in selected regions of the brain. The center of these plaques are composed mainly of fibrillary aggregates of a common, but not well understood, b amyloid peptides (Aβ). The Aβ peptides are generated from the larger amyloid-β precursor protein (APP) by the sequential action of β- and γ-secretase, and it is generally accepted that oligomeric forms of this Aβ are neurotoxic, resulting in disease progression.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service