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MilliporeSigma

AB1557

Sigma-Aldrich

Anti-NMDAR2B Antibody, pain

serum, Chemicon®

Synonym(s):

N-methyl D-aspartate Receptor Subtype 2B, NR2B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

suitability

not suitable for immunohistochemistry

NCBI accession no.

NM_000834.2

UniProt accession no.

Q13224

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GRIN2B(2904)

General description

180 kDa

Immunogen

C-Terminal Fusion Protein of the NMDAR2B (Amino acids 984-1104).

Application

Anti-NMDAR2B Antibody, pain detects level of NMDAR2B & has been published & validated for use in IP & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blotting 1:200-1:1,000 (NMDAR2B is present in high concentrations in the hippocampus)


Immunoprecipitation using solubilized hippocampal slices. 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain lysate.


Not suggested for use in immunohistochemistry.


Note: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.


Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes NMDAR2B. By Western blot it recognizes a 180 kDa band in rat brain membranes. Also recognizes a 70 kDa band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption of AB1557 with the C-terminal fusion protein that was used to generate the antibody.

Physical form

Rabbit antiSerum. Liquid, no preservatives.
Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of methylmercury on postnatal neurobehavioral development in mice.
Yu Gao,Yu Ding,Rong Shi,Ying Tian
Neurotoxicology and Teratology null
Kevin K Ogden et al.
PLoS genetics, 13(1), e1006536-e1006536 (2017-01-18)
N-methyl-D-aspartate receptors (NMDARs), ligand-gated ionotropic glutamate receptors, play key roles in normal brain development and various neurological disorders. Here we use standing variation data from the human population to assess which protein domains within NMDAR GluN1, GluN2A and GluN2B subunits
Y Honse et al.
Neuroscience, 122(3), 689-698 (2003-11-19)
N-methyl-D-aspartate receptor dysfunction has been strongly suggested to link with the abnormalities seen in fetal alcohol syndrome. Thus, the effects of prenatal ethanol exposure on the total expression of NR1 splice variants and the cell surface expression of both NR1
Elisa A Waxman et al.
The Journal of biological chemistry, 280(32), 29322-29333 (2005-06-22)
N-methyl-d-aspartate receptor (NMDAR) stimulation activates many downstream mechanisms involved in both cell survival and cell death. The manner in which the NMDAR regulates one of these pathways, the p38 mitogen-activated protein kinase (p38) pathway, is currently unknown. In the present
Integrin signaling cascades are operational in adult hippocampal synapses and modulate NMDA receptor physiology.
Joie A Bernard-Trifilo, Eniko A Kramar, Reidun Torp, Ching-Yi Lin, Eduardo A Pineda et al.
Journal of Neurochemistry null
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Glutamate is an excitatory neurotransmitter found in the synaptic vesicles of glutamatergic synapses. The post-synaptic neurons in these synapses contain ionotropic and metabotropic glutamate receptors. Glutamate binds to AMPA (α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid) subtype glutamate receptors, leading to sodium influx into the post-synaptic cell and resulting in neuronal excitability and synaptic transmission. The NMDA (N-methyl-d-aspartate) subtype glutamate receptors, on the other hand, regulate synaptic plasticity, and can influence learning and memory. The metabotropic g-protein coupled mGluRs modulate downstream calcium signaling pathways and indirectly influence the synapse’s excitability. The synaptic architecture includes intracellular scaffolding proteins (PSD-95, GRIP), intercellular cell adhesion molecules (NCAMs, N-Cadherins), and a variety of signaling proteins (CaMKII/PKA, PP1/PP2B). Processes critical for synaptic transmission and plasticity are influenced by these molecules and their interactions. When the function of these molecules is disrupted, it leads to synaptic dysfunction and degeneration, and can contribute to dementia as seen in Alzheimer’s disease.

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