17-500M
Catch and Release v2.0 Reversible Immunoprecipitation System
Synonym(s):
Catch and release system, Immunoprecipitation kit
Sign Into View Organizational & Contract Pricing
Select a Size
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NB.22
species reactivity (predicted by homology)
all
Quality Level
manufacturer/tradename
Catch And Release
Upstate®
technique(s)
activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
Related Categories
General description
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Application
Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
Packaging
17-500A is supplied as 5 immunoprecipitations per kit.
17-500 is supplied as 50 immunoprecipitations per kit.
17-500 is supplied as 50 immunoprecipitations per kit.
Preparation Note
All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Analysis Note
routinely evaluated by immunoprecipitation.
Other Notes
Catch and Release Wash Buffer, 10X
Catch and Release Non-denaturing Elution Buffer, 4X
Catch and Release Denaturing Elution Buffer, 1X
Catch and Release Capture Tubes
Catch and Release Non-denaturing Elution Buffer, 4X
Catch and Release Denaturing Elution Buffer, 1X
Catch and Release Capture Tubes
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
E-Y Huang et al.
Cell death & disease, 3, e251-e251 (2012-01-13)
Galectin-1 is a lectin recognized by galactoside-containing glycoproteins, and is involved in cancer progression and metastasis. The role of galectin-1 in radiosensitivity has not previously been investigated. Therefore, this study tests whether galectin-1 is involved in the radiosensitivity mediated by
Enhanced protein recovery and reproducibility from pull-down assays and immunoprecipitations using spin columns.
A Brymora et al.
Analytical biochemistry, 295(1), 119-122 (2001-07-31)
Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.
Roman M Salasznyk,Robert F Klees,Adele Boskey,George E Plopper
Journal of Cellular Biochemistry null
INFLUENCE OF GLUCOSAMINE ON GLOMERULAR MESEANGIAL CELL TURNOVER: IMPLICATIONS FOR HYPERGLYCEMIA AND HEXOSAMINE PATHWAY FLUX.
James LR, Le C, Scholey JW
American Journal of Physiology. Endocrinology and Metabolism null
Niall J Lennon et al.
The Journal of biological chemistry, 278(50), 50466-50473 (2003-09-25)
Mutations in the dysferlin gene cause limb girdle muscular dystrophy type 2B and Miyoshi myopathy. We report here the results of expression profile analyses and in vitro investigations that point to an interaction between dysferlin and the Ca2+ and lipid-binding
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service