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MilliporeSigma

17-295

Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.41

Key Documents

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Quality Level

100

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Related Categories

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Analysis Note

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Other Notes

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding.
Tonia R Wooldridge, Laimonis A Laimins
Virology null
Bovine Foamy Virus Transactivator (BTas) Interacts with Cellular RelB to Enhance Viral Transcription.
Wang J, Tan J, Guo H, Zhang Q, Jia R, Xu X, Geng Y, Qiao W
Journal of virology null
Chromatin immunoprecipitation for identifying transcription factor targets in keratinocytes.
Kori Ortt,Satrajit Sinha
Methods in Molecular Biology null
D Pannell et al.
The EMBO journal, 19(21), 5884-5894 (2000-11-04)
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence
Orexin signaling mediates the antidepressant-like effect of calorie restriction.
Lutter, M; Krishnan, V; Russo, SJ; Jung, S; McClung, CA; Nestler, EJ
The Journal of Neuroscience null
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