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Cellvento® 4HEK Powder

Chemically defined, powder, suitable (for HEK293 cell culture)

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About This Item

UNSPSC Code:
41106213
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Quality Level

500

description

shipped with cooling pack.

Dry powder medium must be supplemented with 2.5 g/L Sodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mM L-Glutamine

product line

Cellvento®

form

dry powder

technique(s)

cell culture | mammalian: suitable

suitability

suitable (for HEK293 cell culture)

storage temp.

2-8°C

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Dry powder medium must be supplemented with 2.5 g/LSodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mML-Glutamine.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Can AAV Continue to Deliver the Promise of Gene Therapy?

The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.

Development of a Novel Cell Culture Medium for AAV Production With Multiple HEK293 Lineages and Process Optimization

Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.

High Performing Platforms for Lentivirus and AAV Manufacturing

Upstream gene therapy optimization maximizes viral vector titers; partner with technology experts for HEK293, HEK293T, and Sf9 cell solutions.

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Global Trade Item Number

SKUGTIN
125195001004065266953176
125195000504065266964318
125195002504065266953183
125195005004065266953190
125195010004065266968903