Skip to Content
MilliporeSigma

07-330-AF488

Sigma-Aldrich

Anti-Partitioning-defective 3 Antibody, Alexa Fluor™ 488 Conjugate

from rabbit, ALEXA FLUOR™ 488

Synonym(s):

Partitioning defective 3 homolog, PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, ASIP, CTCL tumor antigen se2-5, PAR3-alpha, Partitioning-defective 3, Alexa Fluor™ 488 Conjugate

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

monkey, canine, human, rat, frog, mouse

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NP_062565

UniProt accession no.

Q8TEW0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PARD3(56288)

General description

Partitioning defective 3 homolog (UniProt Q8TEW0; also known as ASIP, Atypical PKC isotype-specific-interacting protein, Bazooka, CTCL tumor antigen se2-5, Par-3 family cell polarity regulator alpha, Par-3 partitioning defective 3 homolog, PAR3-alpha, Protein phosphatase 1 regulatory subunit 118) is encoded by the PARD3 (also known as Baz, PAR3, PAR3A, PARD3A, PPP1R118) gene (Gene ID 56288) in human. Par3 is a PDZ (PSD95/Dlg/ZO-1) domain-containing membrane scaffold adapter protein that plays an important role in epithelial cell tight junction (TJ) formation and apico-basal polarity maintenance. Together with PAR6, atypical protein kinase C (aPKC), and the Rho GTPase CDC42, Par3 forms a complex that regulates cytoskeleton re-organization, post-golgi vesicle trafficking, microtubule network polarization, and asymmetric cell division. PAR6 interacts directly with aPKC isoforms iota/lambda and zeta to form the complex core. Recruitment of GTP-bound CDC42 to the PAR6-aPKC core induces aPKC phosphorylation/activation by PAR6, and subsequent PAR3 binding. Another quaternary apical junctional complex (AJC), Willin–Par6-aPKC–Par3, has also been reported. In this case, the complex-associated aPKC functions to phosphorylate ROCK, resulting in ROCK dissociation from the AJC and thereby preventing excessive ROCK-dependent apical constriction.
~180, 150, 100 kDa observed

Immunogen

GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Partitioning-defective 3 in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-330) is shown to be suitable also for Western blotting, immunocytochemistry, and immunohistochemistry applications.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Partitioning-defective 3 Antibody, Alexa Fluor™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Partitioning-defective 3.

Biochem/physiol Actions

Expected to react with all 11 spliced isofoms (UniProt Q8TEW0-1 to Q8TEW0-11).

Physical form

Protein A purified
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry in HUVECs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Partitioning-defective 3 in HUVECs.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Post-Translational Modifications
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

"Mods – modifications – small alterations can have a big impact on form and function. In the motorsports world, stock vehicles are modified to enhance their performance. Modifications, or mods, to the engine, drive train, intake and exhaust components add up to provide phenomenal performance gains that can be measured as horsepower and torque increases, which yield a competitive advantage, and result in reduced run times. In biology, proteins undergo modifications that alter their function. Some proteins require the modifications in order to perform their function effectively, whether it’s a pro-protein that is cleaved to produce an active enzyme, or a protein that is phosphorylated to facilitate a signaling process. Other proteins, such as histones, undergo modifications that regulate gene expression and alter cellular function. There are several post translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination that impact protein function and activity. As a result, the analysis of proteins and their post-translational modifications are particularly important for the study of normal and disease-associated processes. New antibodies to detect phospho Histidines are now available from EMD Millipore."

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service