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24107

Nukol Capillary GC Column

size × I.D. 30 m × 0.25 mm, df 0.25 μm

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About This Item

UNSPSC Code:
41115710
NACRES:
SB.54
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material

fused silica

agency

meets requirements for USP G25 and G35

analyte chemical class(es)

free fatty acids, glycols

parameter

60-200 °C temperature (isothermal), 60-220 °C temperature (programmed)

Beta value

250

df

0.25 μm

technique(s)

gas chromatography (GC): suitable

size × I.D.

30 m × 0.25 mm

matrix active group

Bonded; acid-modified poly(ethylene glycol) phase, Nukol

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
food and beverages
forensics and toxicology
personal care

column type

capillary polar

General description

Application: The incorporation of acid functional groups into the phase lends an acidic character to this column, useful for analyses of volatile acidic compounds. Difficult to analyze carboxylic acids (free fatty acids) can be analyzed with excellent peak shape and minimal adsorption.
USP Code: This column meets USP G25 and G35 requirements.
Phase:
  • Bonded
  • Acid-modified poly(ethylene glycol)
Temp. Limits:
  • 60 °C to 200 °C (isothermal) or 220 °C (programmed)

Application

The Nukol column is used for the analysis ofvolatile short-chain fatty acids (SCFAs). The Nukol column is specificallydesigned for volatile acids, allowing for the effective separation andquantification of these compounds in fecal samples. The GC system with theNukol column facilitates the measurement of SCFA concentrations, which are important metabolic products of gut microbial fermentation.
Reference: David, L., Maurice, C., Carmody, R. et al. Diet rapidly and reproducibly alters the human gutmicrobiome. Nature 505,559–563 (2014)
Nukol capillary GC column may be used for quantitative determination of free volatile fatty acids (FVFA) from dairy products. It may also be used for the determination of the free concentration of valproic acid in human plasma by equilibrium dialysis followed by solid-phase microextraction (SPME) and capillary gas chromatography.

Other Notes

We offer a variety of chromatography accessories including analytical syringes

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Certificates of Analysis (COA)

Lot/Batch Number
42504-04B
42504-04A
42504-03B
42504-03A
42504-02B

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Protocols

游离脂肪酸GC分析

本文将讨论游离脂肪酸分析。

Articles

GC Analysis of C2-C7 Free Fatty Acids on Nukol™

Separation of Propionic acid; Acetic acid; Heptanoic acid; Isobutyric acid; Valeric acid; Isocaproic acid; Butyric acid; Isovaleric acid

Solid-phase microextraction for the determination of the free concentration of valproic acid in human plasma by capillary gas chromatography.
Mette K
Journal of Chromatography. B, Biomedical Sciences and Applications, 673 (2), 299-305 (1995)
Quantitative determination of free volatile fatty acids from dairy products on a Nukol capillary column.
Luciano C.
Journal of Chromatography A, 519 (2), 369-378 (1990)

Global Trade Item Number

SKUGTIN
2410704061838080479