TRUE2
TrueGel3D Hydrogel Kits
FAST-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker
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About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice
shipped in
dry ice
storage temp.
−70°C
General description
The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on PEG non cell-degradable crosslinker. Maleimide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD (TRUERGD-1UM) prior to the crosslinking step.
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
Application
TRUE2-1KT is used to grow cells that don′t attach to hydrogel for survival but rather use their secreted extracellular matrix. It is used to immobilize cells or specimens for imaging and for bioprinting.
Features and Benefits
- Fast gelling (takes few seconds to minutes after addition of crosslinker)
- Allow cell recovery and post-culture analyses
- Flexibility in modulating gel stiffness
- Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)
- Does not allow cell migration
Other Notes
TRUE2-1KT contains lyophilized FAST-DEXTRAN (polymer) and PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol, and water.
Disclaimer
Avoid prolong exposure of PEG non cell-degradable crosslinker to air.
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Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
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Protocols
An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation
TrueGel3D™ hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.
Articles
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TTrueGel3D™是一种用于3D细胞培养的合成水凝胶平台，可模拟天然细胞外环境以获得更符合生理的培养条件
Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| TRUE2-1KT | 04061835539253 |