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SRP0265

PDE4C Active from mouse

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym(s):

Dpde1, Dunce, Phosphodiesterase 4C

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Assay:
≥70% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in baculovirus infected insect cells
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biological source

mouse

recombinant

expressed in baculovirus infected insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

94 kDa

packaging

pkg of 5 μg

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... PDE4C(110385)

General description

Mouse PDE4C (GenBank Accession No. NM_000923), full length, with N-terminal GST tag, MW = 94 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C




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Global Trade Item Number

SKUGTIN
SRP0265-5UG04061832781990