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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SAP.4G5, monoclonal
Application:
IF, IHC
Citations:
4
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
SAP.4G5, monoclonal
form
buffered aqueous solution
mol wt
~55 kDa
species reactivity
bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract, immunofluorescence: 1-2.5 μg/mL using HeLa cells, immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TUBB(203068)
General description
Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology.
Immunogen
synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA
Application
Anti-β-Tubulin I antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Brian Perry et al.
Journal of the Association for Research in Otolaryngology : JARO, 4(3), 329-338 (2003-12-24)
The seven mammalian isotypes of beta tubulin are strikingly similar in amino acid sequence. The differences in isotypic sequence, although small, are nonetheless conserved in evolution, which suggests that they may confer distinct functional roles. If so, such roles should
Tubulin modifications and their cellular functions
Hammond JW, et al.
Current Opinion in Cell Biology, 20(1), 71-76 (2008)
S S Siddiqui et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(8), 2963-2972 (1989-08-01)
We have identified 2 anti-tubulin monoclonal antibodies that bind to 2 different subpopulations of identified neurons in the simple nervous system of the nematode Caenorhabditis elegans. The antibodies also recognize specific tubulin isotypes from C. elegans that were separated by
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAB4200732-100UL | 04061838026583 |