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H8791

Transforming Growth Factor-β3 human

Name: Transforming Growth Factor-&#946;3 human
Brand: SIGMA

HumanKine^®, 95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonym(s):

TGF-β3

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00213054

Biological source:

human

Recombinant:

expressed in HEK 293 cells

Assay:

≥95% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

dimer 25 kDa (non-glycosylated)

Impurities:

≤1 EU/μg

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Properties

Product Name

Transforming Growth Factor-β3 human, TGF-β3, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤1.0 ng/mL EC₅₀

quality

endotoxin tested

mol wt

dimer 25 kDa (non-glycosylated)

packaging

pkg of 5 μg

manufacturer/tradename

HumanZyme

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P10600

storage temp.

−20°C

Gene Information

human ... TGFB3(7043)

Description

General description

TGF-β3 (transforming growth factor-β3) belongs to the TGF β superfamily. The TGFβ3 gene is mapped to human chromosome 14q24.3

Biochem/physiol Actions

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

TGF-β3 (transforming growth factor-β3) regulates lymphocyte proliferation, apoptosis, hematopoiesis and embryogenesis to maintain immune homeostasis. TGF-β plays an important role in cell growth, differentiation, and survival. TGF-β3 specifically promotes chondrogenic differentiation.TGF-β3 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β3 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β isoforms is known to participate in wound healing and tissue fibrosis. TGF-β3 is crucial for tissue restoration and scarless tissue repair. Mutation in the TGFβ3 gene is associated with development of non-syndromic cleft palate only (NS CPO), a rare congenital disease.

Physical form

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Preparation Note

HumanKine TGF-β3 is expressed in human HEK 293 cells as a mature, disulfide linked, non- glycosylated homodimer with a predicted molecular mass of 25 kDa.
This product is lyophilized from a solution of 50mM NaOAc, pH 3.7.

Analysis Note

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c