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About This Item
form
prepacked column
crosslinking
cross-linked
feature
wetted part: no
packaging
pack of 30 pieces
manufacturer/tradename
Cytiva 17-0851-01
storage condition
(0.15% kathon)
parameter
2.5 mL sample volume
technique(s)
liquid chromatography (LC): suitable
bed size
14.5 mm × 50 mm
bed volume
8.3 mL
column I.D.
15 mm
matrix
cross-linked dextran
matrix active group
dextran
particle size
50-150 μm
cleaning
2-13
working range
2-13
suitability
suitable for bioprocess medium
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
Features and Benefits
- Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex® G-25 Medium.
- Flexible protocols allow runs by gravity flow or centrifugation.
- Easy to use and equilibrate; apply sample; elute.
- Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
- Desalt multiple samples in parallel with a desalting capacity >90%.
- Recovery in the range 70 to >95%.
Preparation Note
Legal Information
signalword
Warning
hcodes
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
Articles
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0851-01 | 04061835573127 |
