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G5635

β-Galactosidase from Escherichia coli

Name: β-Galactosidase from <I>Escherichia coli</I>
Brand: SIGMA

Grade VIII, lyophilized powder, ≥500 units/mg protein

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase

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About This Item

CAS Number:

9031-11-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-864-1

MDL number:

MFCD00155434

EC Number:

3.2.1.23 (BRENDA, IUBMB)

Specific activity:

≥500 units/mg protein

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Properties

type

Grade VIII

Quality Level

300

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

465 kDa

does not contain

BSA as extender

composition

Protein, ≥60% biuret

storage temp.

−20°C

Description

General description

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Application

β-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter. It may be used as a positive control protein with anti-β-galactosidase antibodies.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical form

Contains Tris buffer salts and magnesium chloride

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

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