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5006

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol™

Synonym(s):

Collagen Type I, PureCol Collagen

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
Biological source:
bovine
Form:
lyophilized
Technique(s):
cell culture | mammalian: suitable
Assay:
99.9%
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Product Name

PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

biological source

bovine

Quality Level

100

sterility

sterile-filtered

assay

99.9%

form

lyophilized

packaging

pkg of 15 mg

technique(s)

cell culture | mammalian: suitable

binding specificity

Peptide Source: Elastin
, Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

−20°C

General description

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKUGTIN
14340-25MG04061838734808
14340-500MG04061838734815
14340-100MG04061838734792
14340-500M04061837383045
505875000104055977263114
5006-15MG04061832422558
HLMIR195504061836727635
HLMIR195604061836727642
HLTUD195504061836096229
HLTUD195604061836096236