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SCC038

Human Adipose Mesenchymal Stem Cells

Human Adipose Mesenchymal Stem Cells (MSCs) are isolated from human adipose tissue and are cryopreserved at P2 to ensure optimal phenotype and the highest viability and plating efficiency.

Synonym(s):

Fat Mesecnhymal Stem Cells, Human Adipose Derived MSCs, Human Adipose Stromal Stem Cell, Human Adipose Stromal Stem Cells, hADSCs

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About This Item

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
human
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biological source

human

Quality Level

100

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable, cell differentiation: suitable

input

sample type mesenchymal stem cell(s)

shipped in

dry ice

storage temp.

-140 to -196°C

General description

EMD Millipore’s Human Adipose MSCs are isolated from human adipose tissue and are cryopreserved at p2 to ensure optimal phenotype and the highest viability and plating efficiency. Each lot of human adipose MSCs originates from a single donor of human lipoaspirate tissue. These cells proliferate as an adherent cell monolayer and have shown a strong capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes. EMD Millipore’s Human Adipose MSCs uniformly express appropriate MSC markers including the integrin CD29, matrix receptors CD44, CD105 and stromal cell-associated markers CD73, CD90, and CD166. The cells are negative for hematopoietic lineage markers CD14, CD31, CD34, and CD45.

Preparation Note

Human Adipose Mesenchymal Stem Cells should be stored in liquid nitrogen. The cells can be passaged for at least three passages (or more than ten population double times) without significantly affect cell surface marker expression and differentiation potential.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
1) 1X106 viable Human Adipose Mesenchymal Stem Cells: (Catalog No. SCC038). Store in liquid nitrogen.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

10 - Combustible liquids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

间充质干细胞培养方案

有关间充质的信息，特别是间充质干细胞培养实验方案用于间充质干细胞（MSC）分离、扩增和分化的分步细胞培养方案。

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Articles

采用LentiBrite™ 慢病毒生物传感器进行细胞骨架蛋白的荧光活细胞成像

包含β-肌动蛋白、α-微管蛋白和波形蛋白用于细胞骨架结果蛋白活细胞分析的高滴度慢病毒颗粒。

新型无异种和无血清成骨分化培养基中人间充质干细胞的加速骨细胞分化

新型无血清、无异种人间充质干细胞（MSC）骨细胞分化培养基的开发。

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

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Related Content

A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells

We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.

Human Adipose Mesenchymal Stem Cell Kit
Yavuz Emre Arslan et al.
Biotechnology progress, 35(4), e2814-e2814 (2019-04-10)
In this study, we aimed at generating 3-dimensional (3D) decellularized bovine spinal cord extracellular matrix-based scaffolds (3D-dCBS) for neural tissue engineering applications. Within this scope, bovine spinal cord tissue pieces were homogenized in 0.1 M NaOH and this viscous mixture

Global Trade Item Number

SKUGTIN
SCC03804053252905827