17-10521
EZ-Magna NuCLEAR™ RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit
EZ-Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.
Synonym(s):
Magnetic RNA-BP Immunoprecipitation, RNA-Binding Protein Immunoprecipitation
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About This Item
Quality Level
manufacturer/tradename
Magna Nuclear RIP
technique(s)
RIP: suitable, activity assay: suitable (protein interaction), immunoprecipitation (IP): suitable
shipped in
dry ice
General description
- Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs
- Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 5,000 cells
- Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
- Suitable for analysis by RT-qPCR or RIP-seq
- Complete set of reagents and detailed protocol to enable first-time success
Magna Nuclear RIP Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq).
Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Cross-linked can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. In contrast native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. For less well understood proteins and protein complexes often both approaches are used.
The kit described here is for the cross-linked approach. If a native approach is of interest please visit the product page for the Magna Nuclear RIP (Native) Kit, catalogue # 17-10522 or the EZ-Magna Nuclear RIP (Native) Kit, catalogue # 17-10523.
Packaging
Physical form
Preparation Note
Kit components are stable for 6 months from date of shipment when stored as directed.
Other Notes
10X PBS
Nuclei Isolation Buffer
RIP Cross-Linked Lysis Buffer
Protein A/G Magnetic Beads
Nuclear RIP Dilution Buffer
Low Salt Wash Buffer
High Salt Wash Buffer
LiCl Wash Buffer
TE Buffer
RIP Elution Buffer
10% SDS
0.5 M EDTA
DNase I (RNase Free)
DNase I Supplement
DNase I Reaction Buffer
Protease Inhibitor Cocktail III, Animal Free
RNAse Inhibitor
Proteinase K
Control Antibodies and Primers
Normal Mouse IgG Negative Control Antibody
Anti-EZH2 Positive Control Antibody
NEAT1 Positive Control Primers
U1 snRNA Negative Control Primers
Legal Information
signalword
Danger
Storage Class
10-13 - German Storage Class 10 to 13
wgk
WGK 3
target_organs
Respiratory Tract
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Inhalation
Certificates of Analysis (COA)
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Related Content
"Gene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. While the regulation of gene expression by transcription factors and epigenetic influences has been well studied over time, pervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored. Chromatin is typically thought of as a complex of DNA, histones, and non-histone proteins, and RNA. Historically, mRNA was considered to be the only RNA associated with chromatin. These mRNAs would transiently associate with chromatin during transcription then exit the nucleus for translation. However, mounting evidence suggests that various classes of non-coding RNAs (e.g. long non-coding RNAs (lncRNA) small nuclear RNAs (snRNA), enhancer RNAs (eRNA) etc.) are associated with chromatin and likely serve regulatory functions1-3. For the past several years chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. The need to better understand the RNA component of chromatin has driven the development of additional methods to allow analysis and characterization of chromatin associated RNA. One approach used to detect and identify RNA molecules that interact with a specific protein is RNAbinding protein immunoprecipitation (RIP)4. This method allows the immunoprecipitation of protein:RNA complexes that are both nuclear and cytoplasmic using whole cell lysates generated using kit such as the Magna RIP™ RNA Binding Protein Immunoprecipitation Kit."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 17-10521 | 04053252984648 |