V900893
Collagenase from Clostridium histolyticum
Type IV, Vetec™, reagent grade, powder, ≥160 CDU/mg solid
Synonym(s):
Clostridiopeptidase A
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-582-9
MDL number:
Specific activity:
≥160 CDU/mg solid
Biological source:
Clostridium histolyticum
biological source
Clostridium histolyticum
grade
reagent grade
product line
Vetec™
form
powder
specific activity
≥160 CDU/mg solid
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
storage temp.
−20°C
General description
Collagenase from Clostridium histolyticum is a collagenolytic enzyme. It interacts with collagen-like substances and is responsible for the degradation of collagen into small peptides.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type IV has low tryptic activity. It is suitable for the digestion of islet cells from the pancreas.
Suitable for use in preparation of single cell suspension for sequencing.
Suitable for use in preparation of single cell suspension for sequencing.
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Analysis Note
Also contains clostripain, neutral protease, and tryptic activities.
Other Notes
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Legal Information
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Reversibility of experimental rabbit liver cirrhosis by portal collagenase administration.
Jin B, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 85(8), 992-992 (2005)
Telomerase reverse transcriptase acts in a feedback loop with NF-KB pathway to regulate macrophage polarization in alcoholic liver disease.
Wu X Q, et al.
Scientific Reports, 6, 18685-18685 (2016)
Xiao-Qin Wu et al.
Scientific reports, 6, 18685-18685 (2016-01-05)
Activation of Kupffer cells (KCs) plays a central role in the pathogenesis of alcoholic liver disease (ALD). C57BL/6 mice fed EtOH-containing diet showed a mixed induction of hepatic classical (M1) and alternative (M2) macrophage markers. Since telomerase activation occurs at