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Merck

G005008

HPLC Analysis of Warfarin Enantiomers on Astec® CYCLOBOND I 2000 (UV at 278 nm)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)
mobile phase: [A] acetonitrile; [B] acetic acid; [C] TEA; (100:0.3:0.2, A:B:C)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 278 nm
injection: 2 μL
sample: 2 mg/mL in mobile phase

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
    Warfarin

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