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Merck

P5975

Millipore

Amino-terminal Met-FLAG-BAP™ Fusion Protein

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About This Item

MDL number:
MFCD01632954
UNSPSC Code:
12352202
NACRES:
NA.32

Key Documents

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mol wt

~49 kDa

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Related Categories

Application

Amino-terminal Met-FLAG-BAP™ Fusion Protein is useful for assurance of the functional integrity of ANTI-FLAGÒ M2 and M5 monoclonal antibodies in immunological procedures such as Western blotting, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy and FACS.
Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Amino-terminal Met-FLAG-BAP™ control protein is a 468 amino acid N-terminal Met-FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular weight of 49.4 kDa. The Amino-terminal Met-FLAG-BAP control protein migrates as a 45-55 kDa band by SDS-PAGE depending on the electrophoretic conditions.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK0200
121K9079
098H9047
062K9075
039H9077

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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Pembersihan protein

Teknik penulenan protein, reagen, dan protokol untuk membersihkan protein rekombinan menggunakan kaedah termasuk, pertukaran ion, pengecualian saiz, dan kromatografi afiniti protein.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Data Sheet - P5975
EZview Red Protein A and ANTI-FLAG M2 Affinity Gels

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001

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