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Merck

HYS020

Sigma-Aldrich

HyStem® Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution

Synonym(s):

Cell Culture Scaffold, HyStem Culture Kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Application

HyStem® cell culture scaffold kit has been successfully used to expand human embryonic stem cells (H9s), umbilical cord blood CD23+ stem cells, hepatic stem cells and hepatic progenitor cells.

Features and Benefits

Customizable and free of animal components, Hystem hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Animal Component Free - As a synthetic Hyaluronic acid-based matrix, Hystem offers complete control of the cellular environment. All components are well-characterized, consistent, and derived from non-animal sources
  • Biologically accurate - Rich in hyaluronic acid, the Hystem hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
With Hystem, researchers can tailor the incorporation of ECM proteins, cell attachment factors, and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem is recommended for in vivo experimentation.

Hystem offers a minimal number of cell attachment sites. If desired, researchers may incorporate attachment factors of their choice.

Legal Information

Hystem is a registered trademark of BIOTIME, INC

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H315,H317,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM4415
SLCM4414
SLCJ7571
SLCK4828
SLCK4829

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Soon Chul Heo et al.
PloS one, 10(3), e0116698-e0116698 (2015-03-17)
Periostin, an extracellular matrix protein, is expressed in bone, more specifically, the periosteum and periodontal ligaments, and plays a key role in formation and metabolism of bone tissues. Human adipose tissue-derived mesenchymal stem cells (hASCs) have been reported to differentiate
Anjali Yadav et al.
Journal of neuroinflammation, 18(1), 238-238 (2021-10-18)
Epigenetic regulation by histone deacetylases (HDACs) in Schwann cells (SCs) after injury facilitates them to undergo de- and redifferentiation processes necessary to support various stages of nerve repair. Although de-differentiation activates the synthesis and secretion of inflammatory cytokines by SCs
Colin D Paul et al.
Biomaterials, 197, 101-118 (2019-01-15)
Biophysical aspects of in vivo tissue microenvironments include microscale mechanical properties, fibrillar alignment, and architecture or topography of the extracellular matrix (ECM). These aspects act in concert with chemical signals from a myriad of diverse ECM proteins to provide cues

Articles

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

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